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Useful validation of sorted fractions was performed by xenotransplantation, the results of which was coupled with miRNA expression profiling to deliver stem cell-linked miRNA expression profiles.
To find out regardless of whether miRNA are differentially expressed in LSC and HSC, we fractionated sixteen AML individual samples and three lineage-depleted (Lin–) wire blood (CB) samples making use of CD34 and CD38 into four populations and subjected Each individual to world-wide miRNA profiling; the stem mobile written content of every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation measured by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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The LSC frequency was lowered in two of 3 samples upon 126KD (Figures 5C and S5D). With each other, these findings recommend that 126KD creates heterogeneous responses with LSC perform and frequency lowered within a subset of AML individuals.
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